http://www.sciencemag.org/news/2019/04/game-changing-gene-edit-turned-anole-lizard-albino

The mighty genome editor CRISPR isn’t so powerful in lizards and snakes: Never before has it been used to edit the embryos of these reptiles. Now, researchers have come up with a workaround—by editing the immature, unfertilized eggs of brown anole lizards.

Researchers typically edit with CRISPR by injecting it into a single-celled fertilized egg, creating a DNA change that is present in all subsequent cells. But female anoles are a special challenge: They store sperm in their oviducts for long periods, making it difficult to time the introduction of CRISPR to fertilization. They also form eggshells at fertilization, and it’s extremely difficult to insert a needle at that stage without damaging the embryo.